KSCP
APFCP
SIUCP-ETS
NSCRD
SCRS
Search
- Page Path
- HOME > Search
Original Article
- In-vitro Chemosensitivity Test for Colorectal Cancer using an Adenosine-triphosphate-based Chemotherapy Response Assay (ATP-CRA).
- Huh, Jung Wook , Park, Yoon Ah , Sohn, Seung Kook , Choi, Sung Ho
- J Korean Soc Coloproctol. 2007;23(3):172-179.
- DOI: https://doi.org/10.3393/jksc.2007.23.3.172
- 2,736 View
- 16 Download
- 3 Citations
-
Abstract
PDF - Purpose
The adenosine-triphosphate-based chemotherapy response assay (ATP-CRA) is a well-documented and validated technology for individualizing chemotherapy in cancer patients. We evaluate the feasibility of ATP-CRA in colorectal cancer patients. This study will illustrate the assay's success rate, the mean coefficient of variation, and the turnaround time as a validation tool for a chemosensitivity test. Methods: A total of 118 patients, treated by surgery between June 2004 and September 2005 were evaluated for chemosensitivity to seven anticancer agents (5-fluorouracil (5-FU), oxaliplatin, irinotecan, epirubicin, etoposide, gemcitabine, and paclitaxel) by using an ATP-CRA. To allow a comparison between samples, we calculated the chemosensitivity index (CI) based on the percentage cell death rate (CDR, %) at each test drug concentration.
Results
The assay success rate was 85.5% (118/138), and the mean coefficient of variation, indicating intra-assay error level, was 9.2%. CDR measured at a therapeutic peak plasma concentration ranged from 0% to 93.6% with a median of 31.0% for 5-FU, 28.5% for oxaliplatin, 34.0% for irinotecan, 25.0% for epirubicin, 21.0% for etoposide, 22.0% for gemcitabine, and 25.2% for paclitaxel. According to the CI, the most sensitive drug varied from patient to patients 10.9% for 5-FU, 10.9% for oxaliplatin, 24.7% for irinotecan, 11.8% for epirubicin, 22.4% for etoposide, 1.1% for gemcitabine, and 23.3% for paclitaxel. Conclusions: Our data suggest that the ATP- CRA is a feasible in-vitro chemosensitivity test in colorectal cancer and that patients show heterogeneous chemosensitivity. A study evaluating the predictive value of ATP-CRA directed therapy is needed to determine the clinical usefulness of the test. -
Citations
Citations to this article as recorded by
- Correlation between the molecular subtype of breast cancer and thein vitroadenosine triphosphate-based chemosensitivity assay
Jina Chang, Anbok Lee, Jihyun Lee, Woosung Lim, Sun Hee Sung, Byung-In Moon
Journal of the Korean Surgical Society.2013; 84(6): 313. CrossRef - Heterogeneity of Adenosine Triphosphate-Based Chemotherapy Response Assay in Colorectal Cancer - Secondary Publication
Jung Wook Huh, Yoon Ah Park, Kang Young Lee, Seung-Kook Sohn
Yonsei Medical Journal.2009; 50(5): 697. CrossRef - Complete Remission of Unresectable Colon Cancer after Preoperative Chemotherapy Selected by Adenosine Triphosphate-Based Chemotherapy Response Assay
Jung Wook Huh, Yoon Ah Park, Eun Joo Jung, Kang Young Lee, Ji Eun Kwon, Seung-Kook Sohn
Journal of Korean Medical Science.2008; 23(5): 916. CrossRef
- Correlation between the molecular subtype of breast cancer and thein vitroadenosine triphosphate-based chemosensitivity assay
First
Prev- Page of 1
- Next
- Last
TOP
