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Journal of the Korean Society of Coloproctology 2006;22(2):75-80.
Methodologic Evaluation of EGFR Expression in Colorectal Cancer.
Oh, Soo Youn , Yoon, Se Jin , Cheon, Seung Hui , Lee, Ryung Ah , Kang, Bo Young , Lee, Shi Nae , Chung, Soon Sup , Kim, Kwang Ho
1Department of Surgery, College of Medicine, Konkuk University, Chungju, Korea.
2Department of Surgery, College of Medicine, Ewha Womans University, Seoul, Korea. eastgate@ewha.ac.kr
3Ewha Medical Research Institute, College of Medicine, Ewha Womans University, Seoul, Korea.
4Department of Pathology, College of Medicine, Ewha Womans University, Seoul, Korea.
Abstract
PURPOSE
The epidermal growth factor receptor (EGFR) is a one of the transmembrane receptor proteins that play an important role in initiating tumor cell signaling and growth and is regarded as a promising target for cancer therapy. The EGFR expression rate has been reported to vary according to the detection method. The aims of this study were to evaluate the EGFR expression rate of a colorectal carcinoma by using immunohistochemical staining (IHC) and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and to analyze the correlation between these methods.
METHODS
EGFR expression was investigated in tissue sections from 33 patients with a colorectal adenocarcinoma by using IHC and semiquantitative RT-PCR. IHC was performed with antibodies in a 1:40 dilution and a 1:80 dilution. The results of the three detection methods were compared with one another.
RESULTS
The mean age of the patients was 61.9+/-12.2 years, and the male-to-female ratio was 1.2:1. The EGFR expression rates were 93.9% (31/33) in IHC with a 1:40 dilution, 87.9% (29/33) in IHC with a 1:80 dilution, and 66.7% (22/33) in RT-PCR. The result of IHC with a 1:40 dilution significantly correlated with the result of IHC with a 1:80 dilution (Pearson correlation 0.684, P<0.01). There was no correlation between semiquantitative RT-PCR and IHC (1:40 dilution, 1:80 dilution).
CONCLUSIONS
The EGFR expression obtained by using IHC was consistent with different antibody dilutions. The expression rate obtained by using RT-PCR was significantly lower than that obtained by using IHC, and there was no statistical correlation between the expressions of EGFR obtained by using RT-PCR and IHC. A standardization for EGFR detection methods is needed to draw any conclusion concerning their activity in colorectal cancer.
Key Words: Epidermal growth factor receptor (EGFR); Immunohistochemistry; Semiquantitative RT-PCR; Colorectal cancer


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