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Original Article
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J Korean Soc Coloproctol. 2010;26(1):45-52.
DOI: https://doi.org/10.3393/jksc.2010.26.1.45
- Association of RNase3 Polymorphisms with the Risk of Colorectal Cancer.
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Kim, Yong , Kang, Dong Baek , Lee, Jeong Kyun , Lee, Young Hwan , Kang, In Hong , Chae, Soo Cheon , Yang, Yun Sik , Park, Won Cheol
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1Department of Surgery, Wonkwang University College of Medicine, Iksan, Korea. parkwc@wonkwang.ac.kr
2Department of Radiology, Wonkwang University College of Medicine, Iksan, Korea.
3Department of Pathology, Wonkwang University College of Medicine, Iksan, Korea.
4Genome Research Center for Immune Disorders, Wonkwang University College of Medicine, Iksan, Korea.
- Abstract
- PURPOSE: RNase3 is a secretory ribonuclease found in eosinophilic leukocytes and is involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. Generally, high levels of RNase3 have been reported in cases of active asthma and allergic diseases. However, a relationship between RNase3 and colon cancer has not yet been reported. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the risk of colorectal cancer in Korean people.
METHODS
Blood sampling of each group was performed, TaqMan in g.-550A>G, PCR-RFLP in g.371C>G, and high resolution melting (HRM) in g.499C>G were analyzed. As results, the three SNPs, g.-550A>G, g.371C>G, and g.499C>G, in RNase3 and their haplotypes were analyzed.
RESULTS
The genotype and the allele frequencies of RNase3 g.-550A>G and g.371C>G were not significantly associated with increased risk for colon cancer compared to the control group, but the RNase3 g.499C>C genotype was associated with a significantly increased risk for colorectal cancer compared to the control group (P=0.001). Also, the RNase3 g.499C>C genotype was more specifically associated with a significantly increased risk for right colon cancer than left colon cancer (P<0.001). In haplotypes of RNase3 SNPs, g.-550G, g.371C, and g.499G were significantly associated with colorectal cancer (P=0.019): more specifically, left colon cancer and rectal cancer than right colon cancer (P=0.048).
CONCLUSION: The RNase3 g.499C>G polymorphism may have an influence on colorectal cancers and may have a more specific influence on right colon cancer than left colon cancer and on rectal cancer. However, the significance of the RNase3 g.-550A>G and g.371C>G polymorphisms need careful interpretation and require confirmation in larger studies.
Keywords :RNase3;Polymorphism;Colorectal cancer
